Commentary
HLA-E Gene from Ophiocomina Nigra (Echinodermata-Invertebrates): Bioinformatics Data
821
Views & Citations10
Likes & Shares
HLA-E gene from MHC system has been recently discovered, in our laboratory, in Invertebrates. Blasts were performed against different species to find or not homologies. Results were given in the precedent communication.
INTRODUCTION
In 2020, we discovered for the first time, MHC genes in Invertebrates and particularly in Echinodermata [1,2]. More recently, in 2022 a biosynthesis of HLA-E (Class I, MHC) gene from O. nigra was performed [3].
The aim of this work is to analyze HLA-E DNA sequence.
MATERIAL AND METHODS
Starting material: DNA sequence of HLA-E transcriptome
TGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATCACGAGGTCAGGAGATCGAGACCATCCTGGCTAACACAGTGAAACCCCGTCTCTACTAAAAATACAAAAAATTAGCCGGGCGTGGTGGCGGGCGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCGGGAGGCGGAGCTTGCAGTGAGCCGAGATCGCGCCACTGCACTCCAGCCTGGGCGACAGAGCGAGACTCTGTCTCAAAAAAAAAAAAAAAAAAAAAAA
RESULTS AND CONCLUSION
- Blastn original sequence
Database: Standard databases were used
We also optimize for: Highly similar sequences (mega blast)
We recall that Molecule type is DNA
Its query length is 281
We find more than 100 sequences producing significant alignments.
First results appear in the table below Table 1.
- BlastX original sequence: The Molecule type is again DNA with a query length of 281
The Database which is used consists in: non-redundant protein sequences (nr)
We obtain more than 100 sequences producing significant alignments
The table is recapitulated as following in Table 2.
CONCLUSION
Results summarized in the tables show homologies between the Ophiocomina nigra HLA-E gene and various proteins issued from Staphylococcus aureus to human Chromosome 13 which is sometimes implicated in human trisomy We note also a strong homology with Macaca fascicularis.: 91,86% of identity.
Mainly we retain that O. nigra HLA-E gene exists in “its own right” and in its amplification in plasmid [3].
- Leclerc M (2020) Evidence of MHC Class I and Class II Genes in Echinodermata. Proteomics Bioinformatics 2(1): 59-61
- Leclerc M (2021) Biosynthesis « De Novo » of the Ophuirid Ophiocomina Nigra Igkappa Gene. J Clin Class Immunol 1(1): 1-4.
- Leclerc M (2022) to be published.
-
Table 1 -
Table 2
