2476
Views & Citations1476
Likes & Shares
Natural killer/T-cell lymphomas are rare and
aggressive diseases whose development is rapidly fatal. These lymphomas are
associated with Epstein-Barr virus (EBV) in 100% of cases. Recently, a
treatment combining L-asparaginase with conventional chemotherapy has increased
significantly the survival of patients. Our work consists on characterizing EBV
(particularly LMP1 modifications) in the blood of NK lymphoma’s patients to see
if a particular strain is associated with this disease and/or with the
sensitivity to treatment.
We studied samples of 13 patients with NK
lymphoma before treatment’s initiation and of 9 patients before and during
treatment. EBV strain typing was performed on the EBNA2 gene. The entire LMP1
gene of each sample was cloned; 6 clones were sequenced and compared to B95-8
strain for type 1 or to Jijoye strain for type 2. An EMSA was made to test the
ability of different strains to activate the NFkB factor.
Type 1 strain was found in most cases. In comparison
to B95-8, three different strains regarding the LMP1 gene were found: without
deletion, with a deletion of 15 bp or 30 bp. 11 patients harbored a deleted
strain before treatment. Deleted strains seemed to be associated with a poor
life expectancy and a limited or non-efficacy of treatment. For 4 patients, an
association of diverse strains was detected, principally during treatment.
In conclusion, the majority of patients in
this series have a type 1 virus, which is found in the general population.
For the vast majority of patients (17/20),
cloning, sequencing of six different clones has highlighted one type of virus.
Deleted viruses were great majority: they were found in 14/17 patients for type
1, 82.3% and in 100% of case the virus type 2. This finding is important
considering that the virus deleted for the LMP1 gene is more aggressive than
others, as reported in the literature.
QUICK LINKS
- SUBMIT MANUSCRIPT
- RECOMMEND THE JOURNAL
-
SUBSCRIBE FOR ALERTS
RELATED JOURNALS
- Dermatology Clinics and Research (ISSN:2380-5609)
- Journal of Renal Transplantation Science (ISSN:2640-0847)
- International Journal of Clinical Case Studies and Reports (ISSN:2641-5771)
- International Journal of Anaesthesia and Research (ISSN:2641-399X)
- Journal of Cell Signaling & Damage-Associated Molecular Patterns
- Stem Cell Research and Therapeutics (ISSN:2474-4646)
- Oncology Clinics and Research (ISSN: 2643-055X)