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Background: Obesity
is a medical condition in which excess body fat has accumulated to the extent
that it may have an adverse effect on health leading to reduced life expectancy
and or increased health problem. Adipose tissue derived hormone and protein,
e.g. leptin, tumor necrosis factor-alpha, adiponectin, interleukin-6. All these
proteins play important role in lipid metabolism.
Aim: Aim of
present study was to evaluate biochemical parameters for diagnosis of obesity
as well as displaying the role of inflammatory markers in pathogenesis of
obesity.
Materials
and methods: The present study consisted of 50
obese patients and control group included the age of 50 and sex matched. Demographic data and clinical history of each subject was
recorded. Anthropometric measurements were recorded of each subject. The
biochemical parameters such as lipid profile, tumor necrosis factor-alpha and
adiponectin were done.
Results: The results were expressed in mean ± standard
deviation. Comparisons of study group and study group to control group were
done by applying test of difference between two sample means at 5% (p-0.05) and
1% (p-0.01) level of significance. In current study, mean levels of total cholesterol, TG, LDL
were significantly (p<0.01) higher in obese patients as compared to healthy
controls while serum level HDL-C was lower in obesity but not statically
significant. Increased level of tumor necrosis factor-Alpha in serum was seen
in obese patients compared to controls while serum adiponectin level was
significantly lower in obesity patients when compared with healthy control
(p<0.01).
Conclusion: The tumor necrosis factor-Alpha serum levels
were higher while adiponectin levels were lower in obese individuals. Thus the
evaluation of these parameters is a crucial step, towards management of obesity
and it may help in predicting and also early management of the expected
complications.
Keywords: Obesity, Tumor necrosis
factor-alpha, Adiponectin
INTRODUCTION
Obesity is a
leading preventable cause of death worldwide. The prevalence is increasing in
adults and even children. It has been seen that it is the most serious health
problem of the 21st century [1].
Obesity is a medical condition in which excess body fat has
accumulated to the extent that it may have an adverse effect on health, leading
to reduced life expectancy and or increased health problem [2].
Prevalence of
obesity is increasing worldwide. At least 2.8 million people die each year due
to obesity. Obesity is more common among women, low socioeconomic population
due to poor-quality diet, sedentary lifestyle and family history of obesity [3].
This rising prevalence of obesity has aroused the interest of researchers in
exploring adipose tissue metabolism. Adipose tissue derived hormone and protein,
e.g. leptin, tumor necrosis factor-alpha, adiponectin, interleukin-6. All these
proteins play important role in lipid metabolism. In adipose tissue
inflammation or dysfunction, adipokines secretion is altered. Adiponectin is an
adiposity specific protein which modulates the metabolic process like glucose
regulation and fatty acid metabolism. Adiponectin secretion is inhibited by
increasing TNF-alpha-pro-inflammatory cytokines exerting numerous effects in
adipose tissue including lipid metabolism [3,4].
Thus aim of present
study was to evaluate biochemical parameters in diagnosing obesity as well as
displaying the role of inflammatory markers in pathogenesis of obesity and its
related consequences which may help in management of expected complications.
MATERIALS AND METHODS
The present
case control study was conducted in the department of Biochemistry in collaboration
with the department of medicine of DVVPF’s Medical college and Hospital. The
study group consisted of 50 obese patients and control group included people of
age 50 and sex matched healthy controls. Depending upon the BMI, theses subject
were divided into two groups with BMI ≥ 30 kg/m2
and control i.e. non obese group with BMI of 18.0-22.0 kg/m2.
All subjects gave informed consent and the study protocol was approved by
institutional ethics committee.
Exclusion criteria
Patients with
acute infection, myocardial infraction (MI), diabetes mellitus, hypertension
thyroid disease, taking drugs like anti-inflammatory or antibiotics were
excluded from study.
Inclusion criteria
All patients were diagnosed by
physician and their mean body mass index (BMI) was greater than 30 kg/m2.
Definition of anthropomorphic indices of obesity
According to the World Health Organization
guidelines, obesity was defined as a BMI ≥ 30
kg/m2, waist circumference >94 cm in men and 80 cm in women.
Waist hip ratio ≥ 0.90 in men and ≥ 8.5 in women [5].
Sample
collection
After 12 h of overnight fast, 7
ml of blood was collected from each subject by venepuncture with standard blood
collection technique in plane vacutainer and (yucca diagnostic) and sodium
fluoride vial sample were centrifuged at 2500 rpm for 10 min. Serum and plasma
collected and stored at -70° until biochemical studies.
Methodology
Demographic data and clinical
history of each subject was recorded. Anthropometric measurements were recorded
of each subject. The biochemical parameters such as lipid profile, tumor
necrosis factor-alpha and adiponectin were done.
1.
Estimation of lipid profile:
Cholesterol oxidase peroxidase (CHOD-PAP) method was used for estimation of
serum, total cholesterol and HDL-C. Enzymatic glycerol 3-phosphate oxidase
(GPO-PAP) method for serum triglyceride (TG) (kit manufactured by Span
Diagnostic Ltd.) using semi-autoanalyser. LDL-C calculated by using Friedelwald
formula (LDL-C=total cholesterol-TG/5-HDL-C) [6-9].
2.
Serum TNF-alpha:
Determination of TNF-alpha (by Boster Biological Technology Co. Ltd., Wuhan,
China) levels were measured using commercially available enzyme-linked Immunosorbant
Assay ELISA kit [10].
3.
Serum adiponectin: Serum
adinopectin was measured by enzyme-linked immunosorbant assay (Sigma Aldrich)
[11].
STATISTICAL
ANALYSIS
Statistical software SYSSTAT-12
(By Cranes Software, Bangalore) was used to analyze the data. The results were
expressed in mean ± standard deviation (mean ± SD). Data was analyzed by
descriptive statistics as mean, SD. Comparisons of study group and study group
to control group were done by applying the test of difference between two
sample means at 5% (p-0.05) and 1% (p-0.01) level of significance.
Correlation analysis was done by
determining Karl Pearson's correlation coefficient for positive and negative
correlation between various parameters in all groups under study. Correlation
was tested by students ‘t’ test at 5% (p-0.05) and 1% (p-0.01) level of
significance.
RESULTS AND DISCUSSION
Table 1 showed that mean levels of Total
Cholesterol, TG, LDL were significantly (p<0.01) higher in obese patients as
compared to healthy control while serum level HDL-C was lower in obesity but
not statically significant.
Table 1 also illustrated that increased
level of tumour necrosis factor-Alpha in serum was seen in obese patients
compared to controls. While plasma adiponectin level was significantly lower in
obesity patients when compared with healthy control (p<0.01).
DISCUSSION
Obesity is due to excessive fat accumulation
that may impair health resulting from social behavior and environmental and
genetic factors [12]. Obesity and its associated conditions such as insulin
resistance, type 2 diabetes, dyslipidemia, etc. represent major challenges for
basic science and clinical research [13].
During last decade, several studies
demonstrated the important role of adiponectin in the regulation of various
physiological processes including insulin responsiveness, glucose and lipid
metabolism as well as endothelium function, inflammatory response and cytokine
signaling. Thus, this study was designed to explore the relationships of
adiponectin, tumor nerosis factor-Alpha with various anthropometric indexes of
in obese population [14].
In the current study, we studied serum lipid
profile of control and study group. In that serum cholesterol, triglycerides
levels were significantly high in study group. However, HDL-Cholesterol levels
were not significantly decreased. Our results are strongly co-ordinated with
previous research [5]. Obesity in type 2 diabetes mellitus had strong positive
correlation with serum cholesterol and triglycerides [15,16].
In present study, TNF-alpha was significantly
higher in obese patients than in the control group. This result was strongly
matched with one of the recent studies which reported that the TNF-alpha level
was significantly greater in obese group when compared with control group. Ahmet celik et al. [17] has
studied relationship between the new anthropometric obesity parameters and
inflammatory markers in healthy adult men. According to them, both general and
abdominal obesity have a pretendency toward high inflammatory markers.
In this study, serum adiponectin level was significantly lower
in the obese patients as compared to control group. These results were exactly
matched with Marta et al. [18]. According to their study, serum adiponectin
level was decreased in obese subjects. They also found adiponectin in adipose
tissue of obese and normal-weight individuals and finally concluded that,
obesity affects synthesis of adiponectin in subcutaneous adipose tissue. As per
Clarisse Noel et al. [5], there was association of serum adiponectin and leptin
with anthropomorphic indices of obesity in a Sub-Saharan African population.
Adiponectin is associated with reduced adiposity especially abdominal
adiposity, improved insulin sensitivity and lower level of pro-atherogenic
blood lipoproteins [5].
CONCLUSION
The biochemical parameters related to lipid
were significantly altered. The tumor necrosis factor-Alpha serum levels were
higher while adiponectin levels were lower in obese individuals. Obesity is a
pandemic condition that leads to health impairment by increasing the developing
diseases such as diabetes mellitus, cardiovascular diseases. Thus the
evaluation of these parameters is a crucial step, towards management of obesity
and it may help in predicting and also early management of the expected
complications.
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