770Views & Citations
Scalp acupuncture was used to treat many disorders in China since 5 BC and has been re-developed by incorporating traditional Chinese acupuncture with modern knowledge of anatomy and physiology during past a few decades. Scalp acupuncture is characterized by inserting needles, at a low angle of approximately 15-30 degrees, into the thin layer of loose tissue beneath the scalp surface of 14 therapeutic lines or zones. In 1991, scalp acupuncture points have been standardized following the announcement by World Health Organization the International Standard Nomenclature for Scalp Acupuncture Points. It is believed that scalp acupuncture is more effective in treating brain-related conditions because stimulating different parts of the scalp by scalp acupuncture closely corresponds to relevant function areas of brain. In this paper, recent development of scalp acupuncture application on some neurological disorders such as stroke, Parkinson’s disease and multiple sclerosis are reviewed.
Keywords: Scalp acupuncture, neurological disorders
There are three basic features of scalp acupuncture that differentiate it from body acupuncture. Firstly, treatment zones (14 lines or zones) that have been mapped onto the scalp are associated with body functions and broad body regions, and are based on the ideas of different schools of scalp acupuncture (Liu et al., 2012; Lu, 1991). Secondly, scalp acupuncture is characterized by inserting needle into a thin layer of loose tissue beneath the scalp surface, at a low angle of about 15-30 degrees, with an insertion distance of about 1 cum (approximately one inch for adult) (Liu et al., 2012). Thirdly, the needles in scalp acupuncture are subjected to rapid stimulation, which may be performed a variety of ways including twirling, pulling/thrusting and electro-stimulation (Lu, 1991).
Stroke is the second most common cause of death preceded only by heart attacks, and is the major cause of disability in the western societies (Kong et al., 2010; Li et al., 2012). Stroke occurs when the blood supply to part of the brain is cut off and is mainly caused by ischemic or hemorrhagic. Ischemic stroke is the most common subtype of stroke, accounting for about 80% of all strokes (Kong et al., 2010; Li et al., 2012). Treatment of stroke depends on the type of stroke and which part of brain is affected. Conventional approaches include medication to prevent and dissolve the blood clots and reduce blood pressure, and surgery to remove blood clots, treat brain swelling and reduce the risk of further bleeding in case of hemorrhagic stroke (Prabhakaran et al., 2015). However, people who survived stroke are often left with long-term problems caused by injury to their brains.
Using scalp acupuncture to stimulate the scalp over the arm and leg motor control areas and other function area of the brain can be very effective to treat the paralysis and other sequelae of stroke. Scalp acupuncture was often in combination with medication or rehabilitation training to promote stroke functional recovery (Hao & Hao, 2008). Patients with ischemic stroke of subacute stage recovered better following combination of body acupuncture and scalp acupuncture treatment compared to conventional therapy. It is believed that subacute stage of stroke occurs between 1-6 months after onset of stroke, which is crucial for patient’s long-term survival. Chen et al., (2014) conducted a randomized controlled clinical trial to assess the efficacy of combination of body acupuncture and scalp acupuncture in patients of subacute stroke. One hundred twenty-six patients were randomly divided into acupuncture treatment group (n=61) and conventional treatment group (n=65). Acupuncture was given 5 times a week for total 8 weeks. The Fugl-Meyer scale and NIHSS scale and Barthel index were used to evaluate the motor functioning, balance, sensation, joint functioning and activity of daily living before and during and after acupuncture treatment and follow-up. Assessment after 4-week acupuncture showed a very good improvement compared to baseline judged by all parameters but did not show significant difference from conventional treatment group. At the end of 8-week acupuncture patients demonstrated markedly improvement in all assessments compared to baseline. Further, acupuncture showed a significant functional improvement compared to conventional group at the end of 8-week treatment and 3 month follow-up assessment (Chen et al., 2014). Authors concluded that combination of body acupuncture and scalp acupuncture achieved better clinical efficacy in stroke recovery compared to conventional treatment.
Another randomized clinical study of evaluating combined therapeutic effect of scalp acupuncture and body acupuncture on limb function in subacute stroke patients was conducted (Tang et al., 2012). The Fugl-Meyer assessment (FMA), US National Institutes of Health Stroke Scale (NIHSS) were used to assess the patients' limb function and nerve functional lesion severity before and after the treatment. Acupuncture treatment (n=55) was given daily for 20 days. Control group (n=55) was given route neurological therapies. At the end of treatment, the FMA scores were increased significantly and NIHSS scores decreased considerably in both groups compared with baselines. However, patients in acupuncture group showed a markedly improvement judged by FMA and NIHSS scores compared with control group. There were no significant differences in recurrence rates between two groups at the end of 3 and 6 months' follow-up (Tang et al., 2012). This suggested that scalp acupuncture combined with body acupuncture could improve limb movement function and reduce the nerve function damage in stroke patients.
In a sham-controlled randomized clinical study (Hsing et al., 2012), 62 patients with 18-month post-diagnosis of ischemic stroke were randomly allocated to receive either 10-seesion of scalp electro-acupuncture treatment or placebo treatment. The outcome of the study was monitored by NIHSS scale. The data showed that scalp acupuncture produced a significant functional improvement judged by NIHSS scale compared with sham group. However, there was no significant difference in the Barthel, Rankin functional scales between two groups (Hsing et al., 2012).
Clinical efficacy of scalp electro-acupuncture on post stroke speech disorder was assessed (Jiang et al., 2015). Sixty patients with post-stroke apraxia were randomly divided into scalp electro-acupuncture plus rehabilitation group (n=30) and rehabilitation only control group (n=30). Scalp acupuncture was performed on the dominant hemisphere Broca area on the left cerebrum once daily for 4 weeks. The speech movement program module in the psychological language assessment including the scores of counting, singing scale, repeating phonetic alphabet, repeating monosyllable and repeating disyllable were monitored in patients of the two groups before and after treatment. At the end of the treatment, patients in both group showed obvious improvement in all parameters mentioned above compared with their respective baseline. Patients in scalp acupuncture group showed 100% (30/30) improvement compared with 53% (16/30) improvement in control group (Jiang et al., 2015).
Effect of scalp acupuncture on two different stroke models: spontaneously hypertensive stroke-prone (SHR-SP) rats and focal cerebral ischemia (by middle cerebral artery occlusion, MCAO) rats were assessed using MRI technique (Inoue et al., 2009). It was found that scalp acupuncture rapidly reduced the volume of the vasogenic oedema and promoted neurological function recovery in SHR-SP model. On the other hand, scalp acupuncture had no markedly effect on the cytotoxic odema, vasogenic oedema and neurological dysfunction in MCAO model (Inoue et al., 2009). This implied that scalp acupuncture may be more beneficial for stroke patients with hypertension-caused vasogenic origin than ischaemic origin.
Mechanisms underlying benefits of scalp acupuncture were investigated in both basic science and clinical setting. Very recently, effect and mechanisms of scalp acupuncture on neurological dysfunction of intracerebral hemorrhage stroke rat model was investigated (Liu et al., 2017). Rat model of intracerebral hemorrhage (ICH) received scalp acupuncture at acupoint DU20 through GB7 on the lesion side, for 30 mins, twice a day, from day one of surgery for consecutive 7 days. A group of intracerebral hemorrhage model not receiving scalp acupuncture and a group of sham surgery and a group of naïve were used as controls. Behavioral tests included a composite neurological scale, corner turn test, forelimb placing test, wire hang task and beam walking were conducted at days 3 and 7, followed by biochemical studies, such as western blot analysis and histopathologic examine. The data showed that at day 3 after intracerebral hemorrhage, there was no significant difference of behavioral tests between scalp acupuncture group and ICH. However, at day 7 after surgery, there was a significant improvement of neurological deficits in scalp acupuncture treated group compared with ICH. Biochemical studies showed that brain content of tumour necrosis factor alpha and nuclear factor KappaB protein expression, inflammatory markers, was markedly decreased in scalp acupuncture group compared with ICH and sham groups. The results demonstrated that improved behavioral effects by scalp acupuncture were associated with decreased inflammation in rat model of intracerebral hemorrhage.
The study of the influence of scalp acupuncture on levels of inflammation in patients with acute cerebral infarction (ACl) was conducted to investigate its mechanism underlying improvement of ACI (Wang et al., 2016). A total of 61 patients with ACI were randomly allocated to scalp acupuncture group (n = 31) and control (medication) group (n = 30). Scalp acupuncture stimulation of bilateral MS 6 and MS 7 was performed daily plus medication for 7 days, while patients in control group were given medication only. Clinical neurological dysfunction scales such as NDS, 0-45 points for consciousness, gazing, facial palsy, speech, myodynamia, walking-ability were monitored at the baseline and at the end of scalp acupuncture. Serum levels of inflammation markers, such as high-sensitivity C-reactive protein (hs-CRP), TNF-α, IL-6, and IL-1β, were assessed at the baseline and 3 and 7-day after scalp acupuncture. At the end of 7-day scalp acupuncture, patients showed a significant improvement of the neurological deficits compared with the baseline scores, and there was marked improvement in neurological dysfunction compared with control group. The levels of all inflammation markers were significantly decreased at both 3 and 7-day scalp acupuncture compared with baseline levels. The levels of inflammation makers were significantly lower in scalp acupuncture compared with control group. There was a correlation between the improved neurological deficit scores and decreased serum inflammation markers (Wang et al., 2016).
Together, the studies showed that scalp acupuncture improved neurological functions in both ischemic and hemorrhage models of stroke. Scalp acupuncture is effective in improving neurological deficits of patients with stroke, and it could be an important part of rehabilitation program for stroke recovery. Scalp acupuncture may 1) promote angiogenesis and improve regional energy metabolism (Xie et al., 2016); 2) up-regulate expression of glial cell-line derived neurotrophic factor, possibly promoting proliferation and differentiation of neural stem cells in the focal cerebral cortex and hippocampus (Lu et al., 2016); 3) ease cerebral vascular immune-inflammatory reactions (Zhang et al., 2007; 2009; Wang et al., 2016; Liu et al., 2017); 4) inhibit cerebral cortical apoptosis (Zhang et al., 2009).
At the site of the inﬂammatory lesions, the myelin sheath which insulates the nerve cells is destroyed in the process of demyelination. When myelin is lost, transmission of signals through nerves is slowed down or blocked, resulting in a range of symptoms, including physical, mental and even psychiatric problems (Compston & Coles, 2008). In some cases, the myelin sheaths around axons can be rebuilt on reducing inﬂammation. This process is called remyelination and is performed by oligodendrocytes. However, if there are not enough oligodendrocytes at the lesion site, remyelination will not occur or will be incomplete. Therefore, nerves will carry out their functions through electrical signals in an abnormal pathway, and the axons continue to remain for long periods without damage. The lost myelin sheath can be replaced by scar tissue where it is called MS, multiple means many and sclerosis means scar formation (Reipert, 2004; Vidal-Jordana & Montalban, 2017).
When the axons are damaged, they do not completely lose their function. As the disease progresses, oligodendrocytes and, ultimately, axons are destroyed, leading to a worsening of the symptoms. Common symptoms may include fatigue, vision problems, numbness and tingling, muscle spasms, stiffness and weakness, mobility problems, pain, problems with thinking, learning and planning, depression and anxiety, sexual problems, bladder problems, bowel problems, speech and swallowing difficulties. However, most people with MS only have a few of these symptoms (Reipert, 2004).
Scalp acupuncture has been shown to be a very effective technique for treating MS, because different parts of the brain, such as motor area, sensory area, foot motor and sensory area, balance area, hearing and dizziness area, and tremor area, are stimulated in MS patients, according to the presence of symptoms (Hao & Hao, 2008; Hao et al., 2013). Scalp acupuncture treatment for MS had much success in reducing numbness and pain, decreasing spasms, improving weakness and paralysis of limbs and improving balance (Hao et al., 2013). Many patients also reported that their bladder and bowel control, fatigue and overall sense of well-being significantly improved after treatment (Hao et al., 2013). This technique not only relives symptoms but also increases the quality of life and slows or reverses the progression of physical disability (Hao & Hao, 2008; Hao et al., 2013).
In one of case studies, a 65-year-old male patient who had had MS for 20 years was treated with scalp acupuncture. The motor area, sensory area, foot motor and sensory area, balance area, hearing and dizziness area, and tremor area were stimulated once a week for 10 weeks, then once a month for six sessions. After the 16 treatments, the patient showed markedly improvements. He was able to stand and walk. The numbness and tingling in his limbs gradually declined. His incontinence of urine or dizziness was significantly improved. He was able to return to work full time. This case demonstrates that scalp acupuncture can be a very effective treatment for patients with MS (Hao et al., 2013).
Parkinson's disease (PD) is the second most common progressive neurodegenerative disease and is characterized by the loss of dopaminergic neurons in the substantia nigra of middle brain and subsequent depletion of dopamine in the striatum (Hornykiewicz, 2001; Obeso et al., 2008). The clinical manifestation of PD motor symptoms includes bradykinesia, resting tremor, rigidity of muscles and joints, gait and posture imbalance. Although the general intervention for PD involves pharmacological, physical, or deep brain stimulation therapies (Salat & Tolosa, 2013; Connolly & Lang, 2014), treatment is accompanied by a number of adverse effects such as dyskinesia and motor fluctuations in 50% of patients after 5 years' treatment and in nearly 100% of patients after 10 years' treatment (Olanow & Schapira, 2013).
Scalp acupuncture was used to PD for some time. Two clinical studies were conducted to assess effectiveness of manual scalp acupuncture on PD (Zhang et al., 2002; Yang et al., 2004). In the study by Zhang et al., (2002), 64 patients with PD were recruited and randomly divided into scalp acupuncture plus medication group (n=32) and medication only group (n=32), and scalp acupuncture treatment was applied daily for 30 days. While the study by Yang et al., (2004), 60 patients were allocated into scalp acupuncture plus medication group (n=30) and medication alone group (n=30), and scalp acupuncture treatment was given on one day interval for 90 days. The Webster scale was used in both studies to monitor the outcome. It was reported that scalp acupuncture for 30 days improved many aspects of the conditions but it was not statistically significant compared with medication control group (Zhang et al., 2002); However, 3-moth of scalp acupuncture treatment markedly improved many perimeters of measurements in patients with PD compared with medication only group (Yang et al., 2004).
The protective mechanism of scalp acupuncture was studied with experimental model of PD (Wang et al., 2009a,b; Qi & Wang, 2011). Scalp electro-acupuncture was applied on acupoints GV 20 and EX-HN 5, once a day, 6 days a course, for total 2 courses on PD models. Immunohistochemistry of tyrosine hydroxylase (TH), the rate-limiting enzyme responsible for catalyzing the conversion of the amino acid L-tyrosine to L-3,4-dihydroxyphenylalanine, TUNEL method was used to observe the apoptotic amount, and in situ hybridization detecting the mRNA expression of brain-derived neurotrophic factor (BDNF) and DAT were used to assess the outcome of the scalp electro-acupuncture. It was found that scalp electro-acupuncture treatment significantly increased the area density (AD), numerical density (ND) and integrating optic density (IOD) of the positive neurons of TH in the substantia nigra of PD model, compared with control groups (Jiang et al., 2006; Qi & Wang, 2011). Further, scalp acupuncture markedly elevated the levels of the mRNA expression of BDNF and DAT in substantia nigra of PD model (Wang et al., 2009a), markedly decreased the amount of apoptosis (Wang et al., 2009b), and compared with control groups. This suggested that scalp acupuncture may increase TH+ cells by elevating the synthesis GDNF mRNA, decrease apoptosis and promote the reuptake of dopamine, leading to alleviate parkinsonian symptoms.
Altogether, scalp acupuncture provides an important complementary/alternative treatment approach for improving symptoms of many neurological disorders symptoms. By closely stimulating affected areas of the central nervous system, scalp acupuncture has showed more effective results compared to other acupuncture techniques. The studies mentioned above also demonstrated that scalp acupuncture treatment is safer, more effective, and caused fewer side effects compared with conventional treatment such as medications in the respective conditions.
1. Chen LF, Fang JQ, Wu YY, Ma RJ, Xu SY, et al. (2014) Motor dysfunction in stroke of subacute stage treated with acupuncture: Multi-central randomized controlled study. Zhongguo Zhen Jiu 34: 313-318.
2. Compston A, Coles A (2008) Multiple sclerosis. Lancet 372: 1502-1517.
3. Connolly BS, Lang AE (2014) Pharmacological treatment of Parkinson disease: A review. JAMA 311: 1670-1683.
4. Doi MY, Tano SS, Schultz AR, Borges R, Marchiori LL (2016) Effectiveness of acupuncture therapy as treatment for tinnitus: A randomized controlled trial. Braz J Otorhinolaryngol 82: 458-4565.
5. Ganesh A, Apel S, Metz L, Patten S (2013) The case for vitamin D supplementation in multiple sclerosis. Mult Scler Relat Disord 2: 281-306.
6. Hao JJ, Cheng W, Liu M, Li H, Lü X, et al. (2013) Treatment of multiple sclerosis with Chinese scalp acupuncture. Glob Adv Health Med 2: 8-13.
7. Hao JJ, Hao LL (2008) Treatment of multiple sclerosis by scalp acupuncture. Acupuncture Today 9: 1-6.
8. He CD, Lang BX, Jin LQ, Li B (2014) Attention deficit hyperactivity disorder treated with scalp acupuncture and EEG biofeedback therapy in children: a randomized controlled trial. Zhongguo Zhen Jiu 34: 1179-1183.
9. He K, Zhang H, Wu QM, Yan J, Shi ZE, et al. (2012) The combined application of scalp and body acupuncture by stages for low limb dysfunction of patients with apoplexy. Zhongguo Zhen Jiu 32: 887-890.
10. Hornykiewicz O (2001) Chemical neuroanatomy of the basal ganglia – normal and in Parkinson’s disease. J Chem Neuroanat 22: 3-12.
11. Hsing WT, Imamura M, Weaver K, Fregni F, Azevedo Neto RS (2012) Clinical effects of scalp electrical acupuncture in stroke: A sham-controlled randomized clinical trial. J Altern Complement Med 18: 341-346.
12. Huang Y, Zhuo Y, Jiang XM, Tang AW, Li DJ, et al. (2009) Effect of scalp acupuncture on regional cerebral blood flow in Parkinson's disease patients. Chin J Integr Tradit West Med (Chin) 24: 305-308.
13. Huang Y, Jiang X, Zhuo Y, Wik G (2010) Complementary acupuncture in Parkinson’s disease: A spect study. Int J Neurosci 120: 150-154.
14. Inoue I, Fukunaga M, Koga K, Wang HD, Ishikawa M (2009) Scalp acupuncture effects of stroke studied with magnetic resonance imaging: Different actions in the two stroke model rats. Acupunct Med 27: 155-162.
15. Jiang XM, Huang Y, Li DJ, Tang AW, Wang SX, et al. (2006) Effect of electro-scalp acupuncture on cerebral dopamine transporter in the striatum area of the patient of Parkinson's disease by means of single photon emission computer tomography. Zhongguo Zhen Jiu 26: 427-430.
16. Jiang XM, Huang Y, Zhuo Y, Gao YP (2006) Therapeutic effect of scalp electroacupuncture on Parkinson disease. J South Med Univ (Chin) 26: 114-116.
17. Jiang Y, Yang Y, Xiang R, Chang E, Zhang Y, et al. (2015) Clinical study of post-stroke speech apraxia treated with scalp electric acupuncture under anatomic orientation and rehabilitation training. Zhongguo Zhen Jiu 35: 661-664.
18. Kong JC, Lee MS, Shin BC, Song YS, Ernst E (2010) Acupuncture for functional recovery after stroke: A systematic review of sham-controlled randomized clinical trials. CMAJ 182: 1723-1729.
19. Kostoff RN, Briggs MB, Lyons TJ (2008) Literature-related discovery (LRD): Potential treatments for multiple sclerosis. Technol Forecast Soc Change 75: 239-255.
20. Li LX, Luo P, Wang Q, Xiong L (2012) Electroacupuncture pretreatment as a novel avenue to protect brain against ischemia and reperfusion injury. Evid Based Complement Altern Med 2012: 1-12
21. Li SK (2014) Effects of scalp acupuncture combined with auricular point sticking on cognitive behavior ability in patients with vascular dementia. Zhongguo Zhen Jiu 34: 417-420.
22. Liu H, Sun X, Zou W, Leng M, Zhang B, et al. (2017) Scalp acupuncture attenuates neurological deficits in a rat model of hemorrhagic stroke. Complement Ther Med 32: 85-90.
23. Liu Z, Guan L, Wang Y, Xie CL, Lin XM, et al. (2012) History and mechanism for treatment of intracerebral hemorrhage with scalp acupuncture. Evid Based Complement Altern Med 2012: 895032.
24. Lu S (1991) Scalp acupuncture therapy and its clinical application. J Trad Chin Med 11: 272-280.
25. Lu L, Zhang XG, Zhong LL, Chen ZX, Li Y, et al. (2016) Acupuncture for neurogenesis in experimental ischemic stroke: A systematic review and meta-analysis. Sci Rep 6: 19521.
26. MacPherson H, Altman DG, Hammerschlag R, Li Y, Wu T, et al. (2010) Revised Standards for Reporting Interventions in Clinical Trials of Acupuncture (STRICTA): Extending the CONSORT statement. Acupunct Med 28: 83-93.
27. Moher D, Hopewell S, Schulz KF, Montori V, Gotzsche PC, et al. (2010) CONSORT 2010 explanation and elaboration: Updated guidelines for reporting parallel group randomised trials. J Clin Epidemiol 63: e1-e37.
28. Obeso JA, Marin C, Rodriguez-Oroz C, Blesa J, Benitez-Temiño B, et al. (2008) The basal ganglia in Parkinson's disease: Current concepts and unexplained observations. Ann Neurol 64: S30-46.
29. Olanow CW, Schapira AH (2013) Therapeutic prospects for Parkinson disease. Ann Neurol 74: 337-347.
30. Prabhakaran S, Ruff I, Bernstein RA (2015) Acute stroke intervention: A systematic review. JAMA 313: 1451-1462.
31. Qi XJ, Wang S (2011) Effects of penetration therapy with scalp electroacupuncture on gene expressions of nerve growth factors in substantia nigra of rats with Parkinson's disease. Zhongguo Zhen Jiu 31: 435-440.
32. Reipert B (2004) Multiple sclerosis: A short review of the disease and its differences between men and women. J Men’s Health Gend 1: 334-340.
33. Salat D, Tolosa E (2013) Levodopa in the treatment of Parkinson's disease: Current status and new developments. J Parkinsons Dis 3: 255-269.
34. Tang X, Tang CL, Xu FM, Xie HW, Li LM, et al. (2012) Effect of scalp acupuncture combined with body acupuncture on limb function in subacute stroke patients. Zhen Ci Yan Jiu 37: 488-492.
35. Vidal-Jordana A, Montalban X (2017) Multiple sclerosis: Epidemiologic, clinical and therapeutic aspects. Neuroimaging Clin N Am 27: 195-204.
36. Wang JH (2016) Effect of scalp-acupuncture treatment on levels of serum high-sensitivity C-reactive protein, and pro-inflammatory cytokines in patients with acute cerebral infarction. Zhen Ci Yan Jiu 41: 80-84.
37. Wang S, Jiang H, Qu L (2009a) Study on the mechanism of electroacupuncture scalp point penetration therapy in action on apoptosis in the Parkinson's disease rat model. Zhongguo Zhen Jiu 29: 309-313.
38. Wang S, Qi XJ, Han D (2009b) Effect of electroacupuncture scalp point-through-point therapy on the expression of tyrosine hydroxylase and dopamine transporter mRNAs in substantia nigra of Parkinson's disease model rats. Zhongguo Zhen Jiu 29: 391-394.
39. WHO (1991) Scientific Group on International Acupuncture Nomenclature. A proposed standard international acupuncture nomenclature. Report of a WHO scientific group. Geneva: World Health Organization.
40. Xie C, Gao X, Luo Y, Pang Y, Li M (2016) Electroacupuncture modulates stromal cell-derived factor-1α expression and mobilization of bone marrow endothelial progenitor cells in focal cerebral ischemia/reperfusion model rats. Brain Res 1648: 119-126.
41. Yang Y, Chen HT (2004) Clinical observation on the treatment of Parkinson’s disease by scalp. JCAM 20: 36.
42. Zhang HX, Liu LG, Zhou L, Huang H, Li X, et al. (2007) Effect of scalp acupuncture on inflammatory response in rats with acute cerebral ischemia-reperfusion injury. J Chin Integr Med 5: 686-691.
43. Zhang HX, Wang Q, Zhou L, Liu LG, Yang X, et al. (2009) Effects of scalp acupuncture on acute cerebral ischemia-reperfusion injury in rats. J Chin Integr Med 7: 769-774.
44. Zhang WG, Wang GB, Qin Y (2002) Scalp acupuncture for treatment of Parkinson disease: 32 cases. Guangming J Chin Med (Chin) 17: 55-56.
- Journal of Blood Transfusions and Diseases (ISSN:2641-4023)
- Advance Research on Endocrinology and Metabolism (ISSN: 2689-8209)
- Journal of Rheumatology Research (ISSN:2641-6999)
- Journal of Otolaryngology and Neurotology Research(ISSN:2641-6956)
- International Journal of Internal Medicine and Geriatrics (ISSN: 2689-7687)
- International Journal of Diabetes (ISSN: 2644-3031)
- Journal of Oral Health and Dentistry (ISSN: 2638-499X)