Abstract
Newly Synthesised Fluorescence Iron Chelators with Cytotoxic Effects and In Vitro, In Vivo Imaging Possibilities
Tereza Brogyanyi*, Veronika Antonyová, Robert Kaplánek, Jakubek Milan, Jan Pankrác and Martin Vokurka
Corresponding Author: Tereza Brogyanyi, BIOCEV (Biotechnology and Biomedicine Centre of the Academy of Science and Charles University in Vestec), First Faculty of Medicine, Charles University, Kateřinská 32, 12108 Prague 2, Czech Republic
Accepted: November 01, 2019
Citation: Brogyanyi T, Antonyová V, Kaplánek R, Milan J, Pankrác J, et al. (2019) Newly Synthesised Fluorescence Iron Chelators with Cytotoxic Effects and In Vitro, In Vivo Imaging Possibilities. BioMed Res J, 3(S1): 31.
Copyrights: ©2019 Brogyanyi T, Antonyová V, Kaplánek R, Milan J, Pankrác J, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Iron is an essential element for humans and most other organisms. It is crucial for processes such as oxygen transport, energetic metabolism (oxidative phosphorylation), detoxification of chemicals, synthesis of DNA and many others. On the other hand, the excess of free iron can participate in oxidative stress reactions with the production of free radicals (Fenton’s chemistry) and may cause severe tissue damage. That is why both systemic and intracellular iron levels must be tightly regulated. Iron, not surprisingly, plays an important role in many pathological conditions like neurodegenerative diseases and cancer.

The project was focused on cancer study and was divided in two parts- chemical and biological. First step was concentrate on characterization of complexation possibilities newly synthesised chelators. The titrations with ferric and ferrous ions were performed. The chelators with highest affinity to irons ions were chosen for advanced studies. The project covers in vitro cytotoxic studies on different types of tumor cell lines (MiaPaca, Patu, Capan), MTT assay was used to establish IC 50. Cytotoxic effect of chelators on tumor cell lines was compared with cytotoxicity on human fibroblasts (healthy cell lines). Imaging possibilities of fluorescent chelators were studied as well. The fluorescent chelators were able to cross cellular membranes, in concentration of 1uM and thus provide us the image of their intracellular transport, subcellular localization and intracellular redistribution. For in vivo imaging nu/nu mice with suppressed immune system were used. The distribution of fluorescence chelators after administration was visualized by Xtreme device (Brucker).

 

Keywords: Iron, Chelators, Imaging, Tumor diseases, Cytotoxicity