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In this study Out of 280 urinary tract
infections samples, 212 (75.7%) bacterial isolates were recovered. Based on,
morphological, cultural and biochemical testes, further, confirmed the results
by VITEK 2 System there were 54 (30.2%) of gram positive whereas the 158
(69.8%) of gram negative. The bacterial isolates were distributed as Escherichia coli followed by Klebsiella pneumoniae, Staphylococcus aureus, Pseudomonas aeruginosa and Staphylococcus epidermidis which
represented 96 (45.2%), 48 (22.6%), 43 (20.3%), 14 (6.6%) and 11 (5.2%)
respectively. The synthesis of zinc oxide nanoparticles (ZnO NPs) using
biological methods as eco-friendly from the use of Aspergillus niger filtrate in the extracellular synthesis, then
identified and characterized by UV-Vis spectrophotometer and Scanning Electron
Microscopy (SEM). Furthermore, determine the antibacterial efficacy of
biological synthesized ZnO NPs against isolated pathogens microbes Staphylococcus aureus and Escherichia coli. The results found the
used of 100 mL of ZnCl2 in cultivation of Aspergillus niger was obtained and biosynthesis of ZnO NPs at 46
mg. SEM was illustrate the morphology and practices of ZnO NPs sizes and appear
as spherical in shape besides, the size range at 41-75 nm. The assurance by the
UV-Vis spectrum appears the absorption bands of ZnO NPs at 380 nm. The results
of 1.0 and 1.5 mg mL-1 from ZnO NPs synthesized from A. niger were effect of inhibition
against S. aureus and E. coli bacterial isolated from urine
tracts infection sources. The Inhibition Zone Diameters (IZD) against S. aureus was appear at 24 and 26 mm,
respectively compared with the IZD from ciprofloxacin alone at 20 mm. While the
same concentration from ZnO NPs against E.
coli causing in IZD at 25 and 28 mm, respectively, compared with
Ciprofloxacin inhibition alone at 23 mm.
Keywords: Zinc oxide NPs, Aspergillus
niger, SEM, Antibacterial activity
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