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Pylorus is considered one of the most
etiologies of active chronic gastritis, peptic ulcer, ulcer and inflammation of
the duodenum, gastric cancer and tumors associated with the mucous layer. This
study was done since there is a shortage in such local study in this concern.
The study conducted the Isolation and diagnoses of Helicobacter pylori bacteria biopsy from 80 cases taken by the
endoscope from patients in Tikrit Teaching Hospital, Iraq. Two biopsy samples
were taken from each patient of the mucus layer of the antrum to investigate
pylorus. The first sample was used for Rapid Urease Test (RUT), while the
second sample was used for culturing on its selective media. Seventy three (73)
samples which represent 91.25% of total samples were shown positive result for pylorus.
These bacterial isolates were orally administration
for mice as infection trials, later some of these infection trials were treated
with probiotic bacteria (Lactobacillus
plantrum) as therapy and protection treatments. To demonstrate the vital
and histological influences in the tissue of stomach of laboratory mice as well
as determine the inhibitory influence of their impact in the cases of oral
administration of probiotics type Lactobacillus
plantrum. The study showed that the efficiency of the rapid urease test for
the investigation of the H. pylori bacteria,
as it was able to prove the presence of the bacteria in all positive samples
taken. The catalase and oxidase tests were also showed positive results for all
the 73 samples. In addition, these samples showed positive in culture media
under micro aerophilic conditions. The results also showed inflammation changes
in the stomach tissues of the mice infected with the H. pylori bacteria. The effectiveness of therapy treatment by
probiotic bacteria (Lactobacillus
plantrum) was very obvious by inhibition of the negative effect to a large
extent in all measured standards has reached the state of gastric tissue in
which case results resemble to the control group.
Keywords: Pylorus, Probiotic bacteria, Histological influences, Rapid urease
test
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