|Medinat Ronke Suleiman*, Joan Ejembi, Fatimah Jummai Giwa, Olarenwaju Jimoh, Abdulmajeed Olayinka Suleiman and Adebola Tolulope Olayinka|
|Corresponding Author: Medinat Ronke Suleiman, Department of Medical Microbiology, Faculty of Basic Clinical Sciences, Ahmadu Bello University, Zaria, Nigeria|
|Published: August 10, 2019;|
|Citation: Suleiman MR, Ejembi J, Giwa FJ, Jimoh O, Suleiman AO, et al. (2019) Serotype Distribution Pattern of Streptococcus pneumoniae Isolates from Invasive Infections at a University Teaching Hospital in Northern Nigeria. J Immunol Res Ther, 4(S1): 13.|
|Copyrights: ©2019 Suleiman MR, Ejembi J, Giwa FJ, Jimoh O, Suleiman AO, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
Background: Infections with Streptococcus pneumoniae are endemic worldwide. It is a public health problem and responsible for 1.6 million of 8.8 million annual deaths of children under 5 years of age, with 50% occurring in sub-Saharan Africa. This descriptive study was done to determine the prevalent S. pneumoniae serotypes responsible for infections at Ahmadu Bello University Teaching Hospital, Shika-Zaria, Nigeria.
Materials and methods: Clinical specimens of blood, cerebrospinal fluid and aspirates from abscess, ear swab, throat swab, pus and sputa were collected over a period of 18 months from 420 patients with pneumonia, meningitis, septicemia and otitis media. Specimens were cultured on 5% defibrinated sheep blood agar and chocolate agar. Incubation was done aerobically in a CO-enriched atmosphere at 37°C for 18-24 h.
Isolates of S. pneumoniae were identified by standard biochemical techniques using Gram reaction, catalase test, Optochin disc and bile solubility tests. Antimicrobial susceptibility was determined by the modified Kirby-Bauer disc diffusion method with Mueller-Hinton agar supplemented with 5% sheep blood. Serotyping was done using the slide agglutination method (Denka Seiken Co. Ltd., Japan). The serotype final results were recorded as matching, discordant or non-typeable.
Results: A total of 420 patients participated in this study, in which 227 were males (54%) and 193 were females (46%). Participants’ ages ranged from 2 days to 85 years. S. pneumoniae isolates were mainly from blood 12 (52.2%) and sputum 6 (26.1%). Samples with most isolates were from the pediatric age group of 15 years (65.2%). The serotypes identified were 6, 19 and 20 which were all from blood, as none of the strains from sputum was typeable.
Conclusion: The major S. pneumoniae serotypes found in this study were 6, 19 and 20.
Keywords: Capsule, Serotype, Streptococcus pneumoniae, Northern Nigeria
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