Abstract
Cloning and Sequencing of Blood Protein LMP1 in Lymphoma NK Patients
Mohamad Adnan Halabi*
Corresponding Author: Mohamad Adnan Halabi, Medical Laboratory Department, Faculty of Health, Holy Family University, Batroun, Lebanon
Published: August 10, 2019;
Citation: Halabi MA. (2019) Cloning and Sequencing of Blood Protein LMP1 in Lymphoma NK Patients. J Immunol Res Ther, 4(S1): 05.
Copyrights: ©2019 Halabi MA. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
 

Natural killer/T-cell lymphomas are rare and aggressive diseases whose development is rapidly fatal. These lymphomas are associated with Epstein-Barr virus (EBV) in 100% of cases. Recently, a treatment combining L-asparaginase with conventional chemotherapy has increased significantly the survival of patients. Our work consists on characterizing EBV (particularly LMP1 modifications) in the blood of NK lymphoma’s patients to see if a particular strain is associated with this disease and/or with the sensitivity to treatment.

We studied samples of 13 patients with NK lymphoma before treatment’s initiation and of 9 patients before and during treatment. EBV strain typing was performed on the EBNA2 gene. The entire LMP1 gene of each sample was cloned; 6 clones were sequenced and compared to B95-8 strain for type 1 or to Jijoye strain for type 2. An EMSA was made to test the ability of different strains to activate the NFkB factor.

Type 1 strain was found in most cases. In comparison to B95-8, three different strains regarding the LMP1 gene were found: without deletion, with a deletion of 15 bp or 30 bp. 11 patients harbored a deleted strain before treatment. Deleted strains seemed to be associated with a poor life expectancy and a limited or non-efficacy of treatment. For 4 patients, an association of diverse strains was detected, principally during treatment.

In conclusion, the majority of patients in this series have a type 1 virus, which is found in the general population.

For the vast majority of patients (17/20), cloning, sequencing of six different clones has highlighted one type of virus. Deleted viruses were great majority: they were found in 14/17 patients for type 1, 82.3% and in 100% of case the virus type 2. This finding is important considering that the virus deleted for the LMP1 gene is more aggressive than others, as reported in the literature.