Review Article
Breast Milk, Formula Milk, Cow Milk, Soy Milk and Malaria
Pierre Lutgen* and Constant Tchandema
Corresponding Author: Pierre Lutgen, IFBV-BELHERB, Luxembourg, Lubumbashi, Congo, E-mail: lutgenp@gms.lu
Received: May 20, 2019; Revised: November 10, 2019; Accepted: May 25, 2019
Citation: Lutgen P & Tchandema C. (2019) Breast Milk, Formula Milk, Cow Milk, Soy Milk and Malaria. J Pharm Drug Res, 2(6): 195-202.
Copyrights: ©2019 Lutgen P & Tchandema C. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Mother’s milk is known for centuries to be beneficial for infants and to prevent many diseases, including malaria. What are the constituents of breast milk responsible for this efficacy? Do formula milk, cow milk or soy milk has same properties?

 

Keywords: Mother’s milk, Formula milk, Infants, Diseases, Cow milk, Soy milk

A FEW HISTORICAL DATA

It all begins in 1952 with the work of the Liverpool School of Tropical Medicine [1,2].

They found that in rats inoculated with Plasmodium berghei and living on a diet of milk there was a strong suppression of the growth of the parasites. This was valid for whole cow’s milk, reconstituted dried milk from different origins and human milk. Most rats on normal laboratory diet died in a few days. The authors suggested that the milk might contain an inhibitory substance and that herein lies the explanation of the common observation that severe malaria is not often seen in very young infants. In these first experiments only blood-transmitted malaria had been studied.

The London School of Tropical Medicine followed-up on this early work and confirmed that a milk diet had a suppressive action on Plasmodium cynomogi in monkeys. But after a return to normal diet a severe recrudescence took place [3].

In a more recent study, 137 infants exclusively breast fed and 358 control infants from the Democratic Republic of the Congo were assessed for fever and malaria infections by polymerase chain reaction, at 6 months of age. Breast feeding was significantly associated with a reduced risk of clinical malaria [4].

The World Health Organization now recommends exclusive breastfeeding for the first 6 months of life.

NOMADS AND TROPICAL DISEASES

Fulani are a widely spread African ethnic group characterized by lower susceptibility to Plasmodium falciparum and clinical malaria morbidity. They are characterized by a higher rate of lactase persistance. This trait is common in Europe and certain African people with traditions of raising cattle. Lactase non-persistance in other African tribes is often called lactase intolerance. The potential immunoprotective properties of dietary cow milk as a reason for the malaria resistance of Fulani warrant further investigation. [5]

Milk-drinking African nomads show an unusual freedom from infection with Entamoeba histolytica compared with similar nomads taking a mixed diet. The authors related this to a low content in iron in cow’s milk. A personal communication from Dr. Patrick Ogwang informs that in Uganda malaria is highest in East and North Uganda where the staple food is cereals with high iron content, in western Uganda where milk and low iron foods are eaten most malaria is low. In the past however, people in East and North also kept cows (zebu) and took milk regularly and malaria was not as rampant.

One of the first mistakes of Western medicine in Africa was the iron supplementation to the Somali nomads in 1968. Blood analysis of these nomads had shown that according to European standards they were suffering from anemia [6].

The incidence of infections was studied in 137 iron deficient Somali nomads, 67 of whom were treated with placebo and 71 with iron. Seven episodes of infection occurred in the placebo group and 36 in the group treated with iron; these 36 episodes included activation of pre-existing malaria, brucellosis and tuberculosis. This difference suggested that host defence against these infections was better during iron deficiency than during iron repletion.

In an outbreak of Plasmodium falciparum malaria following re-feeding after famine cerebral malaria was restricted to children eating grain. Those given grain were more likely to experience cerebral malaria. Nomad children consuming a predominantly milk diet were free of this complication despite an equivalent incidence of uncomplicated malaria. Freedom of nomads from cerebral complications may be due to inhibition by the milk diet of rapid division of the parasite combined with delayed recovery after famine of T cell function [7].

Some early results

Formula-fed babies contract gastroenteritis more than breast-fed babies, which is of concern to mothers who cannot breastfeed or, as with HIV-infected mothers, are discouraged from breastfeeding. The ability of endogenous breast milk xanthine oxidase to generate the antimicrobial radical nitric oxide has been measured and its influence on the growth of Escherichia coli and Salmonella enteritides examined. Breast milk, but not formula feed, generated nitric oxide. Xanthine oxidase activity substantially inhibited the growth of both bacteria. An important natural antibiotic system is missing in formula feeds; the addition of xanthine oxidase may improve formula for use when breastfeeding is not a safe option [8].

Work done on the shores of Lake Victoria suggested that milk might be deficient in certain nutrients required by the parasite, but that these are present in any normal diet. For the first three months in life children are exclusively breast-fed and they stay malaria free. At the third or fourth month mothers usually start giving them a variety of foods in addition to the breast milk and these extras might supply the nutrients required by the parasite.

In 1983 a five month study was undertaken in Brazil to test the effect of a total milk diet on the susceptibility of mice to various doses of a the rodent malaria P. berghei. The development of humoral immunity was followed by quantitation of the specific serum immunoglobulins (IgG and IgM). High levels of IgG antibodies persisted for 150 days, IgM antibodies were only observed during the two first weeks of infection. The results indicated that a milk diet administered to mice as the only source of food protected them against fatal malaria infection regardless of the number of parasites inoculated. The acquired immunity was still present in the mice at 150 days post inoculation. [9,10].

But these very promising treatments were ignored by BigPharma and subsidized research. Obviously dairy milk or dried milk is not an interesting cash cow in the fight against malaria.

The detrimental role of para-aminobenzoic acid (PABA)

In the light of resistance to most, if not all, of the pharmaceutical molecules (chloroquine, amodiaquine, lumefantrine, chloroquine, sulfadoxine-pyrimethmine) some research groups have tried to better understand all these fifty years old findings. And the proposed detrimental role of PABA (p-aminobenzoic acid) seems to be confirmed. PABA is a precursor of folic acid.

A large scale clinical trial was run on 25 000 infants in Pemba, Zanzibar. The iron and folic acid-containing groups of the trial had to be stopped on the recommendation of the data and safety monitoring board. It resulted in an increased risk of severe illness and death [11-15].

The National Institute for Medical Research finds that milk contains very little p-aminobenzoic acid (PABA), not more than 0.004 ppm [16].

This substance is much more plentiful in some of the constituents of a good laboratory diet, where the vegetal components contain up to 60 ppm of PABA. In vitro Plasmodium requires PABA for satisfactory growth. Experiments were undertaken to study this possibility. Rats were maintained on different diets: normal diet, milk, milk with 1000 ppm of PABA. Only the rats on the milk diet stayed free of Plasmodium berghei on day 12. Plasmodium requires exogenous dietary PABA for survival [17].

Plasmodium generates its own PABA in its apicomplexan organelle, but not in quantities sufficient to guarantee survival and multiplication of trophozoites and schizonts in the infected erythrocyte. And as the human body does not generate its own PABA or folates, but takes them essentially from green vegetables, the parasite has to rely on this supply. If the diet is exclusively on milk it has no chance to survive.

PABA is an intermediate in the synthesis of folate. And the folate supplements sold in our nutrition might be harmful to African new-borns infected by malaria [18].

Iron, zinc, potassium, selenium

Iron is essential for the survival and multiplication of the Plasmodium parasite. In humans iron deficiency appears to protect against severe malaria while iron supplementation increases risks of infection and disease.

Anemia may even protect against malaria as it was found at the University of North Carolina. Researchers studied the red blood cells of 135 anemic children aged 6-24 months in a malaria-endemic region of The Gambia and confirmed that anemia offers greater natural protection against blood-stage malaria infection than sickle-cell trait [19].

It would thus be advisable to keep the iron concentrations rather on the low side. This can be achieved by drinking milk. Lactoferrin, a glycoprotein found in milk, has the ability to bind Fe ions with high affinity and to regulate iron distribution within the body [20].

Zinc is an essential element with strong bactericidal properties and very efficient against diarrhea and other diseases. UNICEF estimates that a formula-fed child living in unhygienic conditions is between 6 and 25 times more likely to die of diarrhea and four times more likely to die of pneumonia than a breastfed child. Zinc stimulates the immune system and increases CD4. Most medical plants like Artemisia are rich in zinc [21].

This might be one of the reasons why human milk is rich in zinc. The zinc content of milk varies with species, lower in cow milk, and stage of lactation, much higher in colostrum. This is probably contributing to the immunity of new-borns against malaria. Variations in zinc absorption from different milks and formulas employed in infant feeding are of serious concern [22].

There is considerable evidence to suggest that the bioavailability of zinc from human milk is especially favorable. It has been confirmed with radioactive zinc studies in adults in whom absorption with mature human milk averaged 57% compared with 32% for cow’s milk. Hence, zinc plasma concentrations of infants fed with cow’s milk-based infant formula was significantly lower. Zinc absorption from soy-based infant formulas is especially poor. The poor absorption of zinc from soy formulas has been found to be attributable to the phytate present in these formulas.

Potassium concentrations in mother’s milk are 2 times higher at postpartum in colostrum than one month later in mature milk. It is likely that potassium plays a key role. The potassium concentration in the plasma of neonates is much higher than in the plasma of the mothers: 5.9 mmol/l versus 3.8 mmol/l [23,24].

The content of selenium in colostrum is significantly higher (28.6 ng/ml) than that in mature milk (15.1 ng/ml) [25].

Fats, taurine, linoleic acid, oxidants

Mother’s milk is rich in fats: 4.4 % versus 3.3% in bovine milk. A fatty diet kills the sporozoites in the hepatocytes by mediating oxidative stress [26].

And rich in linoleic acid, a strong antimalarial. Linoleic may also act as a growth promotor in the neonate. Concentration was quantified in human milk and infant formula. Concentration of the biologically important conjugated linoleic acid in human milk ranged from 2.23 to 5.43 mg/g; that of formula from undetectable to 2.04 mg/g fat [27,28].

Arachidonic acid and docosahexaeonic acid, n-3 and n-6 long chain polyunsaturated fatty acids, are well present in mother’s milk but are absent from many infant formulas. During neonatal life, there is a rapid accretion of arachidonic and docosahexaenoic acid in infant brain. Cognitive development of breast-fed infants is generally better [29-32].

Arachidonic acid also has strong antimalarial properties via PGE production. Already in 2000 it had been demonstrated in a study on Gabonese children with and without malaria that prostaglandins are important pro-inflammatory mediators of the host-immune response to infection [33].

The concentration of arachidonic acid is on the average 0.5% by wt in breast milk. The IUPAC Lipid Handbook confirms that human milk contains arachidonic acid, but cow's milk does not [34-37].

Arachidonic acid is much higher in breast milk than in formula milk or bovine milk [38,39].

Immunoglobulins

Mother’s milk is rich in taurine: 358 mg/kg. Cow’s milk only contains 50 mg/kg, formula milk 30. Taurine has a strong effect on immunity. Replacement 50% of the sulfated amino acid methionine from plant origin by taurine from mammal origin doubles IgA in broilers and increases IgM by 50% [40-42].

Neonates and young infants up to 6 months are relatively protected against symptomatic malaria. The prevailing paradigm was that maternal antimalarial antibodies transferred to the fetus in the last trimester of pregnancy protect the infant from early infections. However direct evidence and research results do not support this paradigm [43].

The mystery of the invasion of hepatocytes through Kupffer cells may eventually find an answer in this context. Kupffer cells are specialized macrophages and protect the liver against microbes, contaminants and other aggressions. Why these phagocytes are used as entry gate by sporozoites indeed is difficult to understand [44].

Some studies have shown that IgA antibodies preferentially attach to hepatocytes, blocking the entry for sporozoites. Their number on the surface of Kupffer cells is much lower, 10% versus 63% on hepatocytes. If so, it is logical to expect that taurine has prophylactic antimalarial properties [45].

Breast milk is a remarkably “altruistic” secretion, that is, its contents are directed at protecting the infant with minimal benefit to the mother. The concentration of antibodies, mainly IgA, is 10-100 folds higher than in serum. In colostrum it is as high as 90 g/L.

Mother’s milk is rich in hydrogen peroxide in the first postpartum week. Hydrogen peroxide like other ROS kills parasites [46].

Estimation of nitrate and nitrite concentrations of milk sources may provide another insight. In colostrum (1-3 days postpartum) nitrite concentrations are much higher than in mature milk (0.08 mg/100 mL versus 0.001) [47].

According to the authors this change is partly due to the changing intestinal microflora in the baby and the changing metabolic demands as the baby grows. The beneficial effects of NO in adult stomachs on gastroprotective and immunomodulatory functions are known. Arginine plays a key role in the metabolism of nitrates. Therefore, it is reasonable to surmise that nitrite must be supplied to the newborn by colostrum. A recent thesis from Sweden confirms and documents well all these positive elements. Dietary nitrates have potent anti-inflammatory effects, without impairing the ability to clear an infection. They are able to restore the gastric and colonic mucus layer in case of colitis [48].

In breastfed infants “good bacteria” of the gut are important in determining the “direction” of maturation of immunity. Together with other maternal and infant factors, the breastfed infant's mucosal and systemic immune responses are influenced by a different micro eco milieu of the gut compared to the formula fed infant. An environment that does not encourage the hatching of Trichuris trichuria eggs due to the absence of the required ‘pro hatching’ bacteria. Escherichia coli in the gut in the breastfed, is deemed another indirect anti-parasitic potential that lies within breast milk [49].

Several protector mechanisms have been proposed for Lactobacillus against gastroenteritis. The most likely mechanism is its role as immunomodulator. Higher bottle feeding with milk poorer than breast milk in Lactobacillus increases the risk of diarrhea. In a trial probiotics Lactobacillus and Bifidobacterium shortened duration of diarrhea to 34.1 h versus 58 h with placebo and reduced the number of stools (7.3 vs. 15.9 with placebo) [50-53].

Immunoglobulins are much higher in breast milk than in formula or cow milk (Figure 1).

Lactoferrin, the best vaccine against malaria

Breast milk also contains lactoferrin, 5-13 g/L in colostrum and 2-4 g/L in milk. The concentration of lactoferrin in milk varies widely from one species to another. It is highest in human milk (2 g/L, 25x MI, moderate in murine milk (0.28 mg/L, 3.5x M) and very low in ruminant milk (-0.01 mg/L in bovine milk, 0.12 × 10-6 M). There is an international trend toward the addition of lactoferrin to infant formula [54,55].

The bactericidal and bacteriostatic properties of lactoferrin are well known Lactoferrin indeed binds strongly to iron, and almost irreversibly. This complex in a dose dependent manner enhances ROS production. A variety of free radical ions inhibits a variety of tumors, intracellular parasites and microbes.

A lower expression of the multidrug resistant gene (MDR1) is noticed. This can be a helpful in decreasing the resistance mechanisms of pathogens.

In parallel there is a 4-fold increase in phagocytic capacity of macrophages. Mouse peritoneal macrophages or human blood monocytes co-cultured with intracellular forms of Trypanosoma cruzi in the presence of human lactoferrin took up greater numbers of organisms than in the absence of lactoferrin [56,57].

The binding of lactoferrin to iron is 250 times higher than for the parent molecule transferrin and down to a much lower pH. Iron is one on the most important promoters of Plasmodium development [58].

Lactoferrin is to a large extent destroyed at temperatures >60°C. Breast milk is thus by far preferable to sterilized cow milk or powdered milk [59].

Very low density lipoproteins (VLDL), similarly to malaria sporozoites are removed from the blood circulation by the liver within minutes after injection by Anopheles mosquitoes. The sporozoite's surface is covered by the circumsporozoite protein (CS). Lactoferrin, a protein with antibacterial properties found in breast milk is also rapidly cleared from the circulation by hepatocytes in case of malaria infection. CS, lactoferrin and remnant VLDLs compete in vitro and in vivo for binding sites on liver cells [60] (Figure 2).

Other authors also found that lactoferrin inhibits sporozoite invasion liver cells in a concentration-dependent fashion. Up to 80%, this is much higher than the 32% of the GSK-Bill Gates vaccine.

Studies have shown that lactoferrin is the special constituent that allows iron-catalyzed toxic O₂ species to efficiently work their damage. Adding 10-8 M pure lactoferrin has a significant impact on hemolysis. Lactoferrin seems particularly suited to focus its products directly onto membranes of target cells. Lactoferrin is highly cationic, which suggests that it might be readily absorbable to negatively charged cellular membranes of erythrocytes.

Plasmodium falciparum extensively remodels its host red blood cell. The zeta potential is an electrochemical property of cell surfaces that is determined by the net electrical charge of molecules exposed at the surface of cell membranes. The RBC membrane is negatively charged and is surrounded by a fixed layer of cations. Using an electrophoretic mobility assay, it was found that the main zeta potential was significantly lower in in RBCs infected with Plasmodium falciparum [61,62].

Lactoferrin can also be found in honey, generated by the metabolism of bees. Bee sting contains the highest concentration of lactoferrin. It is secreted by the serous cells of the major and minor salivary glands. It has an iron-chelating property which deprives microorganisms of this essential element. In addition, lactoferrin has demonstrated potent antiviral, antifungal and antiparasitic activity, towards a broad spectrum of species. Lactoferrin exhibits in vitro anti-inflammatory activities and several domains are present within its polypeptide chain that demonstrates antimicrobial effects [63].

Mycobacterium tuberculosis and most bacteria, except Borrelia (Lyme), must import iron from its host for survival, and its siderophore-dependent iron acquisition pathways are well established. Lactoferrin extracts excess iron from host proteins.

So maybe the message of Melinda Gates in the Wall Street Journal of May 20, 2016 has a lot of merit “Many newborn deaths can be prevented by simple, inexpensive measures, such as, teaching women to breast-feed, which immediately gives a baby nutrients and hydration, and guards against infection, one of the biggest killers of newborns. Each year 2.9 million infants die in their first 28 days of life.”

But a press release of April 28, 2017 shows that the Gates Foundation supports research into milk to find new drug molecules which enhance the absorption and efficacy of existing drugs. The interest of Bill Gates in milk is only to find new molecules for the BigPharma business. 

1.       Maegraith BG, Deegan T, Sherwood Jones E (1952) Suppression of malaria (P. berghei) by milk. Br Med J 2: 1382-1384.

2.       Keppie Audrey A (1953) Modified course of T. congolense infection in mice given diets with milk casein. Br Med J 2: 853-857.

3.       Bray RS (1953) Effect of milk diet on P. cynomolgi infections in monkeys. Br Med J 1: 1200-1201.

4.       Brazeau NF, Tabala M, Kiketa L (2016) Exclusive breastfeeding and clinical malaria risk in 6 month old infants: A cross-sectional study from Kinshasa, Democratic Republic of the Congo. Am J Trop Med Hyg 95: 827-830.

5.       Lokki I, Järvelä I, Holmberg V (2011) Lactase persistence genotypes and malaria susceptibility in Fulani in Mali. Malaria J 10: 9. 

6.       Murray MJ, Murray AB, Murray MB, Murray CJ (1978) The adverse effect of iron repletion on the course of certain infections. Br Med J 2: 1113-1115.

7.       Murray MJ, Murray AB, Murray NJ, Murray MB (1978) Diet and cerebral malaria: The effect of famine and re-feeding. Am J Clin Nutr 31: 57-61.

8.       Stevens CR, Millar TM, Clinch JG, Kanczler JM, Bodamyali T, et al. (2000) Antibacterial properties of xanthine oxidase in human milk. Lancet 356: 829-830.

9.       Ferraroni JJ (1983) Efeito da dieta lactea na supressao da parasitemia. Mem Inst Oswaldo Cruz 78: 27-35. 

10.    Murray MJ, Murray A, Murray CJ (1980) The salutary effects of milk on amoebiasis and its reversal by iron. Br Med J 280: 1351-1352.

11.    Sazawal S, Black RE, Ramsan M, Chwaya HM (2006) Effects of routine prophylactic supplementation with iron and folic acid on admission to hospital and mortality in pre-school children in a high malaria transmission setting: Community-based, randomised, placebo-controlled trial. Lancet 367: 133-143.

12.    McConkey Glenn A (1999) Targeting the Shikimate pathway in the malaria parasite Plasmodium falciparum. Antimicrob Ag Chemother 43: 175-177.

13.    Kretschmar W, Voller A (1973) Suppression of Plasmodium falciparum malaria in Aotus monkeys by milk diet. Z Tropenmed Parasitol 24: 51-59.

14.    Jacobs RL (1964) Role of p-aminobenzoic acid in Plasmodium berghei infection in the mouse. Exp Parasitol 15: 213-225.

15.    Nowell F (1970) The effect of a milk diet upon Plasmodium berghei, Nuttallia (=Babesia) rodhaini and Trypanosoma brucei infections in mice. Parasitology 61: 425-433.

16.    Hawking F (1954) Milk p-aminobenzoate and malaria of rats and monkeys. Br Med J 1: 425-429.

17.    Kicska Gregory A, Ting LM, SchrammVern L, Kim K (2003) Effect of dietary p-aminobenzoic acid on murine Plasmodium yoelii infection. JID 188: 1776-1781.

18.    Carter JY, Loolpapit MP, Lema OE, Tome JL, Nagelkerke NJ, et al. (2005) Reduction of the efficacy of antifolate antimalarial therapy by folic acid supplementation. Am J Trop Med Hyg 73: 166-170.

19.    Goheen MM, Wegmüller R, Bah A, Darboe B, Danso E, et al. (2016) Anemia offers stronger protection than sickle cell trait against the erythrocytic stage of Falciparum malaria and this protection is reversed by iron supplementation. EBioMedicine 14: 123-130.

20.    Baker Heather M, Edward N (2004) Lactoferrin and iron: Structural and dynamic aspects of binding and release. Biometals 17: 209-216.

21.    Mocchegiani E (2007) Zinc and ageing: Third Zincage conference Immunity & Ageing 4: 5. 

22.    Hambidge K, Casey CE, Krebs NF (1986) In: Mertz W (ed) Trace elements in human and animal nutrition. Academic Press: Orlando FL.

23.    Silprasert A, PPruenglampoo L (1991) Composition of sodium, potassium, calcium, magnesium and phosphorus in human breast-milk at different stages of lactation period. Available at: http://thaiagris.lib.ku.ac.th

24.    Martinerie L, Pussard E, Foix-L'Hélias L, Petit F, Cosson C, et al. (2009) Physiological partial aldosterone resistance in human newborns. Pediatr Res 66: 323-328.

25.    Kim ES, Kim JS, Tamari Y (1998) Quantitation of taurine and selenium levels in human milk. Adv Exp Med Biol 442: 477-486.

26.    Zusarte-Luis V, Mota MM (2017) Dietary alterations modulate susceptibility to Plasmodium infection. Nat Microbiol Lett.

27.    Mc Guire MK, Park Y, Behre RA, Harrison LY, Shultz TD, et al. (1997) Conjugated linoleic acid concentrations of human milk and infant formula. Nutr Res 17: 1277-1283.

28.    Brenna T, Varamini B, Jensen RG (2007) Docosahexaenoic and arachidonic acid concentrations in human breast milk worldwide. Am J Clin Nutr 85: 1457-1464.

29.    Fleith M, Clandinin MT (2005) Dietary PUFA for preterm and term infants: Review of clinical studies. Crit Rev Food Sci Nutr 45: 205-229.

30.    Lauritzen L, Carlson SE (2011) Maternal fatty acid status during pregnancy and lactation and relation to newborn and infant status. Matern Child Nutr 2: 41-58.

31.    Innis SM (2007) Human milk: Maternal dietary lipids and infant development. Proc Nutr Soc 66: 397-404.

32.    Douglas JP, Peter GK, Brice WJ (2001) Inverse relationship of plasma prostaglandin E2 and blood mononuclear cell cyclooxygenase-2 with disease severity in children with Plasmodium falciparum malaria. J Infect Dis 183: 113-118.

33.    Brenna JT, Varamini B, Jensen RG, Diersen-Schade DA, Arterburn LM (2007) Docosahexaeonic and arachidonic acid concentrations in human breast milk worldwide. Am J Clin Nutr 85: 1457-1464.

34.    Kim H, Kang S, Jung BM (2017) Brest milk fatty acid composition and fatty acid intake of lactating mothers in South Korea. Br J Nutr 13: 1-6.

35.    Henjum S, Lie O, Chandyo RK, Kiellevold M (2017) Erythrocyte fatty acid composition of Nepal breast-fed infants. Eur J Nutr 57:1003-1013.

36.    Koletzko B (2016) Human milk lipids. Ann Nutr Metab 69: 28-40.

37.    Sueyoshi Y, Oda H (1963) Comparative study on amounts of polyunsaturated fatty acids in human and cow’s milk. Keio J Med 12: 27-29.

38.    Barreiro R, Regal P (2018) Comparison of the fatty acid profile of Spanish infant formulas and Galician women breast milk. J Physiol Biochem 74: 127-138.

39.    Lv Q, Sun L, Cui Y, Yang J (2017) Effects of replacement of methionine in diets with taurine on growth performance and blood index in broilers. Springer 975: 989-1000.

40.    Erbersdorfer HF, Greulich HG (1984) Determinations of taurine in milk and infant formula diets. Eur J Pediatr 142: 133-134.

41.    Kassim O, Ako-Anai KA, Martin SK (2000) Inhibitory factors in breast milk, maternal and infant sera against in vitro growth of Plasmodium falciparum. J Trop Pediatr 46: 92-96.

42.    Dobbs K, Dent AE (2016) Plasmodium malaria and antimalarial antibodies in the first year of life. Parasitology 143: 129-138.

43.    Pradel G, Frevert U (2001) Malaria sporozoites actively enter and pass through rat Kupffer cells prior to hepatocyte invasion. Hepatology 33: 1154-1165.

44.    Sancho J, Gonzalz E (1986) The importance of the Fc receptors for IgA in the recognition of IgA by mouse liver cells. Immunology 57: 37-42.

45.    Al-Kerwi EA, Al Hashimi AH, Salman AM (2005) Mother’s milk and hydrogen peroxide. Asia Pac J Clin Nutr 14: 428-431.

46.    Hord NG, Ghannam JS, Garg HK, Berens PD (2011) Nitrate and nitrite content of human, formula, bovine and soy milks: Implications for dietary nitrite and nitrate recommendations. Breastfeed Med 6: 393-399.

47.    Jädert C (2014) Diet and inflammation, the role of nitrate and conjugated linoleic acid. Akademisk afhandeling, Thesis Karolinska Institutet, 49 Prameela Kannan Kutty Breastfeeding and risk of parasitic infection - A review. Asian Pac J Trop Biomed 4: 847-858.

48.    Rerksuppaphol S, Rerksuppaphol L (2010) Lactobacillus acidophilus and Bifidobacterium bifidum stored at ambient temperature are effective in the treatment of acute diarrhea. Ann Trop Pediatr 30: 299-304.

49.    Newburg DS, Pickering (1990) Fucosylated oligosaccharides of human milk protect suckling mice from heat-stabile E. coli. J Infect Dis 162: 1075-1080.

50.    Ruiz-Palacios GM, Cervantes LE, Ramos P (1998) Role of human milk lactadherin in protection against symptomatic rotavirus. Lancet 351: 1160-1164.

51.    Sultana R (2015) Comparison of immunoglobulin levels in human milk, cow milk and formula milk. Kaav Int J Sci Eng Technol, pp: 25-46.

52.    Neville MC (2000) Lactoferrin secretion into milk: Comparison between bovine, murine and human milk. J Anim Sci 78: 26-35.

53.    Hamosh M (1998) Protective function of proteins and lipids in human milk. Biol Neonate 74: 163-176. 

54.    Anand N, Kanwar RK (2015) Effect of lactoferrin protein on red blood cells and macrophages: Mechanism of parasite-host interaction. Drug Des Devel Ther 9: 3821-3835.

55.    Lima MF, Kierszenbaum F (1985) Lactoferrin effects on phagocytic cell function. I. Increased uptake and killing of an intracellular parasite by murine macrophages and human monocytes J Immunol 134: 4176-4183.

56.    Shakibei M, Frevert U (1996) Dual interaction of malaria circumsporozoites protein with the low density lipoprotein receptor. J Exp Med 184: 1699-1711.

57.    Ozturkoglu‐Budak S (2016) Effect of different treatments on the stability of lysozyme, lactoferrin and β‐lactoglobulin in donkey's milk. Int J Dairy Technol.

58.    Sinnis P, Thomas EW (1996) Remnant lipoproteins inhibit malaria sporozoite invasion of hepatocytes. J Exp Med 184: 945-954.

59.    Tokumasu F, Graciela RO (2012) Modifications in erythrocyte membrane zeta potential by Plasmodium falciparum infection. Exp Parasitol 131: 245-251.

60.    Vercellotti GM, van Asbeck BS, Jacob HS (1985) Oxygen radical-induced erythrocyte hemolysis by neutrophils: Critical role of iron and lactoferrin. J Clin Invest 76: 956-962.

61.    Lee SB (2016) Antifungal activity of bee venom against Candida albicans. J Pharmacopuncture 19: 45-50.

62.    Sritharan M (2016) Iron homeostasis in Mycobacterium tuberculosis: Mechanistic insights into siderophore-mediated iron uptake. J Bacteriol 198: 2399-2409.

63.    Posey JE, Gherardini FC (2000) Lack of a role for iron in the Lyme disease pathogen. Science 288: 1651-1653.