Abstract
Chemical Constituents of the Goat Margarine and Antibacterial Activity against Bacterial Pathogens in Sudan
Rasha Khalid Abbas*
Corresponding Author: Rasha Khalid Abbas, Department of Chemistry, Faculty of Science and Arts in Mukhwa, University of Albaha, 65931, Al Bahah, Saudi Arabia
Published: August 10, 2019;
Citation: Abbas RK. (2019) Chemical Constituents of the Goat Margarine and Antibacterial Activity against Bacterial Pathogens in Sudan. J Immunol Res Ther, 4(S1): 27.
Copyrights: ©2019 Abbas RK. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
 

The margarine constituents were examined by Gas Chromatography-Mass Spectrometry (GC-MS), it contained 41 compounds, fifteen of them were identified as the major compound, hexadecanoic acid, methyl ester (22.39%), methyl stearate (14.92%), methyl elaidate (13.80%), methyl tetradecanoate (10.74%), capric acid, methyl ester (8.34%), lauric acid (4.52%), methyl octanoate (2.70%), linoleic acid, methyl ester (2.57%), methyl 11-octadecenoate (1.90%), methyl caproate (1.77%), methyl pentadecanoate (1.72%), methyl (8E,11E)-8,11-octadecadienoate (1.70%), heptadecanoic acid, methyl ester, (1.46%), trans-13-Octadecenoic acid, methyl ester (oleic acid) (1.20%), methyl palmioleate (1.00%). The effect of goat margarine, against four different pathogenic bacteria Escherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium and Bacillus cereus, were carried out by using a disc diffusion technique, the highest antibacterial activity was detected against Salmonella typhimurium and the lowest one against Escherichia coli. The antibacterial activity of the antibiotics (Ciprofloxacin, Tetracycline Ceftriaxone, Chloramphenicol and Gentamycin), were tested by the disc diffusion technique and by measuring zones of inhibition, shows that there were differences, among all antibiotic the highest activity of antibiotic against bacteria was due to the action of ciprofloxacin. Ceftriaxone and Tetracycline antibiotic give lowest activity. Among the bacteria the highest inhibition zone by antibiotic against Salmonella typhimurium and the lowest one against Escherichia coli.