Abstract
The Effect of Pre-Treatment with Toll-Like Receptor 4 Antagonist Resatorvid on Methotrexate-Induced Liver Injury in Rats: Histopathological Study
Bassim I Mohammad, Bassim S Ahmed, Alaa F Hassan* and Samer F Hassan
Corresponding Author: Alaa F Hassan, Department of Pharmacy, Al-Mahmoudiya General Hospital, Baghdad, Iraq
Published: August 10, 2019;
Citation: Mohammad BI, Ahmed BS, Hassan AF & Hassan SF. (2019) The Effect of Pre-Treatment with Toll-Like Receptor 4 Antagonist Resatorvid on Methotrexate-Induced Liver Injury in Rats: Histopathological Study. J Immunol Res Ther, 4(S1): 08.
Copyrights: ©2019 Mohammad BI, Ahmed BS, Hassan AF & Hassan SF. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
 

Objective: This research aims to evaluate the histopathological changes after pretreatment with resatorvid against methotrexate induced-liver injury.

Methods: 28 male albino-wistar rats divided into random 4 groups (7 rats in each). Control group: Rats left untreated. Vehicle pre-treated group: Rats were administered dimethyl sulfoxide (DMSO) followed by methotrexate (MTX). Methotrexate treated group: Rats left untreated then administered MTX. Resatorvid pre-treated group: Rats were administered resatorvid followed by MTX. 24 h after the end of treatment, the animals were sacrificed. Liver tissue samples dissected out immediately and fixed in 10% formalin. The traditional procedures (paraffin-embedded method) was used to prepare liver tissue for microscopic evaluation by none alcoholic fatty liver disease (NAFLD) Activity Score Components.

Results: Liver tissue sections of MTX-treated group show moderate-to-severe steatosis of hepatic cells and micro- and macro- hepatocellular fatty degeneration and giant fatty cysts with chronic inflammatory cells infiltration. While liver tissue sections of the resatorvid pre-treated group show moderate hepatic cellular fatty degeneration, with a decreased number of fatty cysts chains and the inflammation disappeared.

Conclusion: Resatorvid hepatoprotective effect against MTX-induced injury was promising throughout resolving the accompanying inflammation and partial restoring histopathological fatty alterations.


Keywords: Liver steatosis, Methotrexate sodium, Resatorvid, TLR4 receptor